5UWZ

Protein 12 with aldehyde deformylating oxygenase activity from Gloeobacter violaceus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.03317
Spacegroup name	P 21 21 2
Unit cell lengths	69.842, 95.424, 39.255
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.360 - 1.250
R-factor	0.14014
Rwork	0.139
R-free	0.16743
RMSD bond length	0.013
RMSD bond angle	1.649
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.400	1.270
High resolution limit [Å]	1.250	1.250
Rmerge	0.045	0.255
Number of reflections	73111
<I/σ(I)>	20.6	2.5
Completeness [%]	99.7	98.3
Redundancy	4.01

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.2	298	10% PEG 3000, 100 mM citrate, 200 mM NaCl