5UWW
Crystal Structure of DEAF1 Peptide in complex with CRM1 K579A mutant-Ran-RanBP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 93 |
Detector technology | PIXEL |
Collection date | 2016-02-19 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 106.043, 106.043, 304.207 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.241 - 2.152 |
R-factor | 0.1865 |
Rwork | 0.186 |
R-free | 0.22600 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.515 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.190 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.090 | |
Rpim | 0.025 | 0.543 |
Number of reflections | 95096 | 4653 |
<I/σ(I)> | 32.2 | 1.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.7 | 13.8 |
CC(1/2) | 0.526 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 295 | 16% PEG3350, 100 mM Bis-Tris, pH 6.4, 200 mM ammonium nitrate, 16 mM HCl |