5UWS
Crystal Structure of X11L2 NES Peptide in complex with CRM1-Ran-RanBP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2014-12-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 106.646, 106.646, 304.628 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.694 - 2.401 |
R-factor | 0.1894 |
Rwork | 0.188 |
R-free | 0.22240 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.479 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.094 | |
Rpim | 0.041 | 0.605 |
Number of reflections | 69614 | 3419 |
<I/σ(I)> | 24.4 | 1.7 |
Completeness [%] | 99.9 | 100 |
Redundancy | 9.4 | 7.3 |
CC(1/2) | 0.672 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 295 | 17% PEG3350, 100 mM Bis-Tris, pH 6.4, 200 mM ammonium nitrate, 10 mM spermine-HCl |