5UWQ
Crystal Structure of CDC7 NES Peptide in complex with CRM1-Ran-RanBP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2015-08-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9795 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 106.392, 106.392, 304.567 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.272 - 2.278 |
R-factor | 0.1863 |
Rwork | 0.185 |
R-free | 0.22470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hb2 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.535 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.320 |
High resolution limit [Å] | 2.280 | 2.280 |
Rmerge | 0.074 | |
Rpim | 0.038 | 0.542 |
Number of reflections | 80110 | 3899 |
<I/σ(I)> | 20.5 | 1.6 |
Completeness [%] | 99.0 | 98.5 |
Redundancy | 5.7 | 5.7 |
CC(1/2) | 0.578 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 295 | 16% PEG3350, 100 mM Bis-Tris, pH 6.4, 200 mM ammonium nitrate, 8 mM HCl |