5UWQ

Crystal Structure of CDC7 NES Peptide in complex with CRM1-Ran-RanBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	93
Detector technology	CCD
Collection date	2015-08-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	106.392, 106.392, 304.567
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.272 - 2.278
R-factor	0.1863
Rwork	0.185
R-free	0.22470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hb2
RMSD bond length	0.002
RMSD bond angle	0.535
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.320
High resolution limit [Å]	2.280	2.280
Rmerge	0.074
Rpim	0.038	0.542
Number of reflections	80110	3899
<I/σ(I)>	20.5	1.6
Completeness [%]	99.0	98.5
Redundancy	5.7	5.7
CC(1/2)		0.578

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	295	16% PEG3350, 100 mM Bis-Tris, pH 6.4, 200 mM ammonium nitrate, 8 mM HCl