5UWO

Crystal Structure of Engineered FMRP-1b NES Peptide in complex with CRM1-Ran-RanBP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	93
Detector technology	PIXEL
Collection date	2016-02-19
Detector	DECTRIS PILATUS3 X 6M
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	106.475, 106.475, 304.314
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.071 - 2.347
R-factor	0.1886
Rwork	0.188
R-free	0.22330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hb2
RMSD bond length	0.003
RMSD bond angle	0.597
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.390
High resolution limit [Å]	2.350	2.350
Rmerge	0.109
Rpim	0.039	0.571
Number of reflections	74260	3658
<I/σ(I)>	19	1.2
Completeness [%]	100.0	100
Redundancy	8.6	7.7
CC(1/2)		0.444

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	295	16% PEG3350, 100 mM Bis-Tris, pH 6.4, 200 mM ammonium nitrate, 12 mM HCl