5UW7
PCY1 Y481F Variant in Complex with Follower Peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-12 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.07814 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 86.947, 59.629, 134.164 |
Unit cell angles | 90.00, 93.12, 90.00 |
Refinement procedure
Resolution | 86.800 - 2.370 |
R-factor | 0.23865 |
Rwork | 0.236 |
R-free | 0.28405 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.324 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 86.900 | 2.381 |
High resolution limit [Å] | 2.370 | 2.373 |
Number of reflections | 55622 | 551 |
<I/σ(I)> | 14 | 2.1 |
Completeness [%] | 99.2 | 96.2 |
Redundancy | 6.2 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 23% PEG 3,350, 0.1 M magnesium chloride, 0.1 M HEPES free acid (pH 7.5), 7 mg/mL protein, Protein was preincubated with 1 mM PSA1 [14-32] |