5UW5
PCY1 H695A Variant in Complex with Follower Peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-02 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | P 1 |
Unit cell lengths | 65.020, 85.614, 137.870 |
Unit cell angles | 87.49, 78.51, 89.44 |
Refinement procedure
Resolution | 44.900 - 2.940 |
R-factor | 0.18843 |
Rwork | 0.186 |
R-free | 0.22742 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.407 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.900 | 2.950 |
High resolution limit [Å] | 2.940 | 2.940 |
Number of reflections | 61150 | 637 |
<I/σ(I)> | 11 | 2.8 |
Completeness [%] | 98.8 | 98.9 |
Redundancy | 3.9 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 282 | 16 % PEG 8,000, 50 mM calcium acetate, 0.1 M sodium cacodylate, 8 mg/mL protein, Protein was preincubated with 1 mM presegetalin A1 [14-32] |