5UW2

Structure of E. coli MCE protein MlaD, periplasmic domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.116
Spacegroup name	C 1 2 1
Unit cell lengths	122.360, 63.820, 91.150
Unit cell angles	90.00, 130.01, 90.00

Refinement procedure

Resolution	33.599 - 2.850
R-factor	0.2406
Rwork	0.239
R-free	0.26480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5uw8
RMSD bond length	0.003
RMSD bond angle	0.737
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.850
Number of reflections	12551
<I/σ(I)>	14.9
Completeness [%]	97.8
Redundancy	3.7
CC(1/2)	0.470

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M zinc acetate, 0.1 M MES pH 6.0, and 15% ethanol