5UW1
Activated state yGsy2p in complex with UDP-galactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-03 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 193.665, 206.446, 205.099 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 145.500 - 3.260 |
| R-factor | 0.1877 |
| Rwork | 0.185 |
| R-free | 0.23025 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nb0 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.380 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 145.500 |
| High resolution limit [Å] | 3.260 |
| Number of reflections | 63020 |
| <I/σ(I)> | 15.3 |
| Completeness [%] | 98.3 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 25 - 30 % Peg 300, 0.1 M Bis-Tris pH 6.1-6.4, 25 mM G-6-P, 10 mM UDP-Gal |
