5UVZ

BRD4 Bromodomain 2 with A-1354689

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-06-22
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	57.267, 73.642, 33.719
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.970 - 1.630
R-factor	0.189
Rwork	0.187
R-free	0.22500
RMSD bond length	0.010
RMSD bond angle	0.930
Data reduction software	XDS (2.11.7)
Data scaling software	SCALA
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall
Low resolution limit [Å]	73.600
High resolution limit [Å]	1.630
Rmerge	0.048
Number of reflections	18441
<I/σ(I)>	19.6
Completeness [%]	100.0
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	Protein Buffer : 10 mM HEPES PH 7.5 100 mM NaCl 5 mM DTT Crystallization : 15 % (v/v) Ethanol Tris PH 7.0