5UVR

The core region of PilO from the type IV pilus system of Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-25
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	.979
Spacegroup name	P 61 2 2
Unit cell lengths	40.810, 40.810, 250.470
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.342 - 1.700
R-factor	0.2347
Rwork	0.231
R-free	0.26740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rjz
RMSD bond length	0.006
RMSD bond angle	0.826
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	35.342
High resolution limit [Å]	1.700
Number of reflections	14759
<I/σ(I)>	10.9
Completeness [%]	99.9
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.2 M NaCl, 0.1 M CAPS pH 10.5, 20% (v/v) PEG 8000), 3% (v/v) DMSO