Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.969 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 78.804, 78.804, 114.885 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.20678 |
| Rwork | 0.206 |
| R-free | 0.23292 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1XQU |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.765 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 16265 |
| Completeness [%] | 98.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20% w/v PEG 3000, 0.2M sodium chloride, 0.1M HEPES, pH 7.5 |
