5UVK
Serial Millisecond Crystallography of Membrane and Soluble Protein Micro-crystals using Synchrotron Radiation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 298 |
Detector technology | PIXEL |
Collection date | 2016-08-16 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.03 |
Spacegroup name | H 3 2 |
Unit cell lengths | 186.400, 186.400, 60.300 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.010 - 3.100 |
R-factor | 0.17723 |
Rwork | 0.172 |
R-free | 0.20474 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ziz |
RMSD bond length | 0.011 |
RMSD bond angle | 2.093 |
Data reduction software | CrystFEL (0.6.2) |
Data scaling software | CrystFEL (0.6.2) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.010 | 3.180 |
High resolution limit [Å] | 3.100 | 3.100 |
Number of reflections | 6280 | |
<I/σ(I)> | 7.6 | |
Completeness [%] | 99.7 | |
Redundancy | 156.4 | |
CC(1/2) | 0.986 | 0.799 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 7 | 277 | 75 mM HEPES pH 7, 20 mM MgCl2, 9 % PEG 3350 |