5UVJ

Serial Millisecond Crystallography of Membrane and Soluble Protein Micro-crystals using Synchrotron Radiation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	298
Detector technology	PIXEL
Collection date	2016-08-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03
Spacegroup name	P 43 21 2
Unit cell lengths	79.100, 79.100, 38.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.000 - 2.050
R-factor	0.23275
Rwork	0.229
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zix
RMSD bond length	0.013
RMSD bond angle	1.306
Data scaling software	CrystFEL (0.6.2)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.100
High resolution limit [Å]	2.050	2.050
Number of reflections	7164
<I/σ(I)>	11.2
Completeness [%]	99.8
Redundancy	873.3	0.4
CC(1/2)	0.978	0.436

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	3	298	18 % (w/v) NaCl, 6 % (v/v) PEG 6000, 1 M sodium acetate pH 3.0