5UVE
Crystal Structure of the ABC Transporter Substrate-binding protein BAB1_0226 from Brucella abortus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-11-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97926 |
Spacegroup name | P 1 |
Unit cell lengths | 82.602, 92.738, 95.124 |
Unit cell angles | 95.19, 110.87, 111.35 |
Refinement procedure
Resolution | 41.783 - 2.500 |
R-factor | 0.2282 |
Rwork | 0.226 |
R-free | 0.27740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ne4 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.955 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 80763 | 4014 |
<I/σ(I)> | 9.13 | 1.63 |
Completeness [%] | 97.8 | 97.8 |
Redundancy | 2.2 | 2.1 |
CC(1/2) | 0.445 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2 M calcium chloride, 0.1 M Tris pH 8.5, 25 %(v/w) PEG4000 |