5UUJ

Streptomyces sahachiroi DNA glycosylase AlkZ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-25
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97857
Spacegroup name	P 65
Unit cell lengths	78.921, 78.921, 139.401
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.461 - 2.299
R-factor	0.1594
Rwork	0.157
R-free	0.20060
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.769
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHENIX (1.9_1692)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.380
High resolution limit [Å]	2.299	4.950	2.300
Rmerge	0.118	0.056	0.481
Rmeas	0.123	0.058	0.502
Rpim	0.035	0.017	0.142
Number of reflections	21107
<I/σ(I)>	7.7
Completeness [%]	96.2	99.8	100
Redundancy	12.3	12.3	12.6
CC(1/2)		0.999	0.964

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	294	18% w/v PEG8000, 10 mM Tris-HCl, pH 7.5, 10 magnesium chloride