5UTS
Carbon Sulfoxide lyase, Egt2 in the Ergothioneine biosynthesis pathway
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97648 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.840, 195.341, 107.791 |
| Unit cell angles | 90.00, 91.32, 90.00 |
Refinement procedure
| Resolution | 49.348 - 2.303 |
| R-factor | 0.2105 |
| Rwork | 0.210 |
| R-free | 0.24100 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.601 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-2000 |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.360 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 170838 |
| <I/σ(I)> | 11.45 |
| Completeness [%] | 91.0 |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 278 | 27% PEG 3350, 0.2M Na formate, 50mM HEPES pH 7.5 |
