5UTN

Kaposi's Sarcoma Herpesvirus Protease in Complex with Allosteric Inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	I 2 2 2
Unit cell lengths	70.356, 95.801, 119.253
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.707 - 1.800
R-factor	0.2006
Rwork	0.199
R-free	0.22870
RMSD bond length	0.005
RMSD bond angle	0.857
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.21)
Phasing software	PHENIX (Phenix 1.8.4 Phaser 2.1)
Refinement software	PHENIX (1.10pre_2100)

Data quality characteristics

	Overall
Low resolution limit [Å]	59.630
High resolution limit [Å]	1.600
Number of reflections	72019
<I/σ(I)>	24.64
Completeness [%]	100.0
Redundancy	14.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	298	0.1M Sodium acetate pH 7.8, 0.88M NaH2PO4/1.32M K2HPO4, 0.2M KCl