5UTM
Mutant Structures of Streptococcus Agalactiae GBS Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.991, 108.258, 90.649 |
Unit cell angles | 90.00, 106.05, 90.00 |
Refinement procedure
Resolution | 87.120 - 1.770 |
R-factor | 0.1974 |
Rwork | 0.196 |
R-free | 0.22000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5jy6 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.173 |
Data reduction software | XDS (Jun 17, 2015) |
Data scaling software | SCALA (0.1.27) |
Phasing software | PHASER (2.7.17) |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 108.260 | 1.800 |
High resolution limit [Å] | 1.770 | 1.770 |
Rmerge | 0.115 | |
Rmeas | 0.134 | |
Rpim | 0.068 | 0.848 |
Number of reflections | 122468 | |
<I/σ(I)> | 10.6 | |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 3.8 | 3.8 |
CC(1/2) | 0.993 | 0.548 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 20-28% PEG4000, 0.1M MES |