5USN
Crystal Structure of Schizosaccharomyces pombe Pot1pC bound to ssRNA/ssDNA chimera (rGrGrUTACGGT)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-05-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.640, 59.800, 66.080 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.339 - 1.900 |
R-factor | 0.1872 |
Rwork | 0.183 |
R-free | 0.22370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4hik |
RMSD bond length | 0.008 |
RMSD bond angle | 1.104 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA (0.5.25) |
Phasing software | PHASER (2.6.0) |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 59.830 | 59.830 | 1.940 |
High resolution limit [Å] | 1.900 | 9.110 | 1.900 |
Rmerge | 0.082 | 0.065 | 0.273 |
Rmeas | 0.093 | 0.075 | 0.306 |
Rpim | 0.042 | 0.037 | 0.135 |
Total number of observations | 61629 | ||
Number of reflections | 13518 | 1284 | |
<I/σ(I)> | 13.1 | ||
Completeness [%] | 99.8 | 91.4 | 100 |
Redundancy | 4.6 | 3.6 | 4.7 |
CC(1/2) | 0.995 | 0.994 | 0.953 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 277 | 100 mM Tris, 0.2 mM sodium formate, 15% PEG 4000 |