5USD
Crystal structure of MccF-like protein (BA_5613) in the complex with aspartyl sulfamoyl adenylate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-05-02 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97945 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.456, 85.808, 124.804 |
Unit cell angles | 90.00, 92.32, 90.00 |
Refinement procedure
Resolution | 32.611 - 2.095 |
R-factor | 0.183 |
Rwork | 0.181 |
R-free | 0.20960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fd8 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.619 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 32.611 |
High resolution limit [Å] | 2.095 |
Rmerge | 0.093 |
Number of reflections | 84529 |
<I/σ(I)> | 10.5 |
Completeness [%] | 92.7 |
Redundancy | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 297 | 0.1 M TRIS, 25% PEG 3350 |