5USB

Crystal Structure of Schizosaccharomyces pombe Pot1pC bound to ssRNA/ssDNA chimera (rGGTTACGGT)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	41.290, 58.010, 65.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.639 - 1.615
R-factor	0.1772
Rwork	0.176
R-free	0.20270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hik
RMSD bond length	0.016
RMSD bond angle	1.488
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER (2.6.0)
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.640	1.700
High resolution limit [Å]	1.615	1.620
Rmerge	0.064	0.305
Rmeas		0.324
Number of reflections	20602	1852
<I/σ(I)>	10.35
Completeness [%]	96.6
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	50 mM Tris, 0.2 mM sodium formate, 20% PEG 8000