5UR8

Human antibody fragment (Fab) to meningococcal Factor H binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.977
Spacegroup name	P 31 2 1
Unit cell lengths	131.900, 131.900, 90.381
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	70.878 - 1.755
R-factor	0.199304846731
Rwork	0.198
R-free	0.22174
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lmj
RMSD bond length	0.006
RMSD bond angle	0.908
Data reduction software	iMOSFLM (7.2.1)
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER (1.10.1_2155)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.900	1.790
High resolution limit [Å]	1.755	1.755
Rmerge	0.155
Rpim	0.070
Number of reflections	89767
<I/σ(I)>	12.6	0.9
Completeness [%]	97.4	97.4
Redundancy	11.2	9.4
CC(1/2)	0.998	0.247

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	57.5% Tacsimate