5UQZ
Structural Analysis of the Glucan Binding Protein C of Streptococcus mutans Provides Evidence that it Mediates both Sucrose-Independent and -Dependent Adherence
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2013-01-13 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 213.098, 48.596, 40.775 |
Unit cell angles | 90.00, 96.29, 90.00 |
Refinement procedure
Resolution | 40.530 - 1.149 |
R-factor | 0.1256 |
Rwork | 0.124 |
R-free | 0.14630 |
Structure solution method | SIRAS |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHELXE |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.170 |
High resolution limit [Å] | 1.150 | 3.120 | 1.150 |
Rmerge | 0.060 | 0.029 | 0.600 |
Total number of observations | 518169 | ||
Number of reflections | 142968 | ||
<I/σ(I)> | 7.5 | ||
Completeness [%] | 97.2 | 97.1 | 95.9 |
Redundancy | 3.6 | 3.8 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 100 mM Bis-Tris pH 6.5, 25% PEG 3350 |