5UQP
The crystal structure of cupin protein from Rhodococcus jostii RHA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 139.448, 139.448, 80.075 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.212 - 2.400 |
| R-factor | 0.1677 |
| Rwork | 0.166 |
| R-free | 0.19960 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.325 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.089 | 0.850 |
| Rpim | 0.021 | 0.194 |
| Number of reflections | 18365 | 899 |
| <I/σ(I)> | 32.3 | 5.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 19 | 20 |
| CC(1/2) | 0.950 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M Potassium Iodide, 20% (w/v) PEG 3350 |
