5UQJ
Structure of yeast Usb1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9786 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 157.850, 157.850, 44.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.917 - 1.800 |
| R-factor | 0.1895 |
| Rwork | 0.186 |
| R-free | 0.22450 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.681 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.917 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.090 | 2.230 |
| Number of reflections | 49463 | 1517 |
| <I/σ(I)> | 23.3 | 1.4 |
| Completeness [%] | 99.4 | 98 |
| Redundancy | 14.6 | 14.1 |
| CC(1/2) | 1.000 | 0.446 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 2 M ammonium sulfate, 0.1 M sodium acetate pH 4.6 |
