5UQG
Crystal Structure of the Catalytic Domain of the Inosine Monophosphate Dehydrogenase from Campylobacter jejuni in the complex with inhibitor p200
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.887, 137.963, 116.548 |
| Unit cell angles | 90.00, 95.99, 90.00 |
Refinement procedure
| Resolution | 35.905 - 2.030 |
| R-factor | 0.1678 |
| Rwork | 0.166 |
| R-free | 0.20340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r7j |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.152 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Number of reflections | 194228 | 9561 |
| <I/σ(I)> | 11.6 | 1.62 |
| Completeness [%] | 99.6 | 98.9 |
| Redundancy | 3.7 | 3.2 |
| CC(1/2) | 0.558 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 5 % (w/v) 2-propanol, 0.1 M HEPES pH 7.5 |
