5UQA
Insulin with proline analog FzP at position B28 in the R6 state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2016-06-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.936, 61.258, 60.390 |
Unit cell angles | 90.00, 111.69, 90.00 |
Refinement procedure
Resolution | 35.528 - 1.310 |
R-factor | 0.15392757289 |
Rwork | 0.152 |
R-free | 0.18211 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ev6 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.103 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | phenix.refine (1.9_1692+SVN) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.500 | 1.326 |
High resolution limit [Å] | 1.310 | 1.310 |
Number of reflections | 74398 | 2587 |
<I/σ(I)> | 1.7 | |
Completeness [%] | 97.6 | |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.3M Tris pH8, 17mM Zinc Acetate, 1% Phenol, 0.3875M Sodium Citrate, 2.1% Acetone |