5UQA

Insulin with proline analog FzP at position B28 in the R6 state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	293
Detector technology	PIXEL
Collection date	2016-06-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	46.936, 61.258, 60.390
Unit cell angles	90.00, 111.69, 90.00

Refinement procedure

Resolution	35.528 - 1.310
R-factor	0.15392757289
Rwork	0.152
R-free	0.18211
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ev6
RMSD bond length	0.006
RMSD bond angle	1.103
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	phenix.refine (1.9_1692+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.500	1.326
High resolution limit [Å]	1.310	1.310
Number of reflections	74398	2587
<I/σ(I)>		1.7
Completeness [%]		97.6
Redundancy		3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.3M Tris pH8, 17mM Zinc Acetate, 1% Phenol, 0.3875M Sodium Citrate, 2.1% Acetone