5UQ2
Crystal structure of human Cdk2-Spy1 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 273 |
Detector technology | PIXEL |
Collection date | 2016-07-05 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 120.910, 120.910, 77.110 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 85.496 - 2.700 |
R-factor | 0.2185 |
Rwork | 0.212 |
R-free | 0.27520 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.756 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 85.500 | 2.830 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.110 | 1.280 |
Number of reflections | 16255 | 2107 |
<I/σ(I)> | 12.6 | 2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 8 | 8 |
CC(1/2) | 0.990 | 0.680 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 1M lithium chloride, 12% PEG 6000, and 0.1M MES pH 6.2 |