5UPW
CryoEM Structure Refinement by Integrating NMR Chemical Shifts with Molecular Dynamics Simulations
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI POLARA 300 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.2 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 5 |
| Number of Particle | 39712 |
| Software | EMAN,IHRSR,RELION,CTFFIND,MDFF,Rosetta |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
