5UPJ
HIV-2 PROTEASE/U99283 COMPLEX
Experimental procedure
Source type | ROTATING ANODE |
Source details | SIEMENS |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Collection date | 1993-02-23 |
Detector | SIEMENS |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.382, 45.281, 133.359 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.300 |
R-factor | 0.189 * |
Rwork | 0.182 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | EARLIER STRUCTURE FROM SAME LABORATORY |
RMSD bond length | 0.016 |
RMSD bond angle | 2.547 |
Data reduction software | XENGEN |
Data scaling software | XENGEN |
Phasing software | MERLOT |
Refinement software | CEDAR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 2.440 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.064 | 0.303 |
Total number of observations | 33887 * | |
Number of reflections | 8579 | |
<I/σ(I)> | 13.4 | 3.55 |
Completeness [%] | 90.0 | 73 |
Redundancy | 4 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | CRYSTALS WERE GROWN AT ROOM TEMPERATURE IN 4-7 UL HANGING DROPS OF EQUAL VOLUMES OF PROTEIN AT 8-10 MG/L AND WELL SOLUTION OF 30-35%(W/V) PEG 4000 IN 0.1 M HEPES AND PH RANGE 6.8 - 7.6., pH 7.1, vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 8-10 (mg/ml) | |
2 | 1 | reservoir | PEG4000 | 30-35 (%(w/v)) | |
3 | 1 | reservoir | HEPES | 0.1 (M) | |
4 | 1 | reservoir | n-butanol | 1-3 (%) |