5UOZ
Insulin with proline analog FyP at position B28 in the T2 state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 78.094, 78.094, 78.094 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.882 - 1.175 |
| R-factor | 0.141607998932 |
| Rwork | 0.141 |
| R-free | 0.15495 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3t2a |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.373 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692+SVN) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.882 | 1.217 |
| High resolution limit [Å] | 1.175 | 1.175 |
| Rmerge | 0.063 | 0.469 |
| Number of reflections | 26219 | |
| <I/σ(I)> | 18.03 | 3.88 |
| Completeness [%] | 98.8 | 89.84 |
| Redundancy | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 293 | 0.3M Tris, 0.5mM Zinc Acetate, 425mM Sodium Citrate |
