5UOJ

THE STRUCTURE OF THE MAP KINASE P38 AT 2.1 ANGSTROMS RESOLUTION

Replaces:  1P38

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-01-11
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	45.756, 84.928, 123.911
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.050 - 2.100
R-factor	0.15674
Rwork	0.154
R-free	0.21420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ERK
RMSD bond length	0.018
RMSD bond angle	1.879
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.300	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.042	0.242
Number of reflections	25393
<I/σ(I)>	20	5.3
Completeness [%]	86.0	65.6
Redundancy	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	18% polyethylene glycol (PEG) 8000, 0.2 M Mg(OAc)2, 0.1 M Hepes, pH 7.0