5UOH
Crystal Structure of Hip1 (Rv2224c) T466A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-03 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 104.249, 104.249, 127.707 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.569 - 2.609 |
| R-factor | 0.2205 |
| Rwork | 0.217 |
| R-free | 0.25790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.152 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 42.600 |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.171 |
| Number of reflections | 24852 |
| <I/σ(I)> | 12 |
| Completeness [%] | 100.0 |
| Redundancy | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 30% (W/V) PEG3350, 0.1 M NaAcetate pH 5.6, 0.2 M (NH4)2SO4, 10% (V/V) glycerol |
