5UNE
Dimerized Structure Gives Further Insight Into the Function of the Novel RNA Gene: HAR1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2009-05-01 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.8550 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.562, 39.826, 80.974 |
Unit cell angles | 90.00, 111.83, 90.00 |
Refinement procedure
Resolution | 39.035 - 2.900 |
R-factor | 0.205628872665 |
Rwork | 0.201 |
R-free | 0.24942 |
Structure solution method | AB INITIO PHASING |
RMSD bond length | 0.006 |
RMSD bond angle | 1.183 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 75.000 |
High resolution limit [Å] | 2.900 |
Rmerge | 0.042 |
Rpim | 0.086 |
Number of reflections | 8346 |
<I/σ(I)> | 7.4 |
Completeness [%] | 99.9 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 500mM Tris-HCl, 1MKCl, 50mM MgCl2 |