5UNC
The crystal structure of PHOSPHOENOLPYRUVATE PHOSPHOMUTASE from Streptomyces platensis subsp. rosaceus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-16 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97926 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 89.326, 122.036, 136.681 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.550 - 1.710 |
R-factor | 0.1563 |
Rwork | 0.155 |
R-free | 0.17730 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.054 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 1.730 |
High resolution limit [Å] | 1.710 | 1.710 |
Rmerge | 0.092 | 0.761 |
Rpim | 0.026 | 0.304 |
Number of reflections | 161349 | 8027 |
<I/σ(I)> | 29.1 | 2.66 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.3 | 7.2 |
CC(1/2) | 0.842 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M Ammonium Tartrate Dibasic, 20% (w/v) PEG 3350 |