5UNC
The crystal structure of PHOSPHOENOLPYRUVATE PHOSPHOMUTASE from Streptomyces platensis subsp. rosaceus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 89.326, 122.036, 136.681 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.550 - 1.710 |
| R-factor | 0.1563 |
| Rwork | 0.155 |
| R-free | 0.17730 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.054 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.730 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmerge | 0.092 | 0.761 |
| Rpim | 0.026 | 0.304 |
| Number of reflections | 161349 | 8027 |
| <I/σ(I)> | 29.1 | 2.66 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.3 | 7.2 |
| CC(1/2) | 0.842 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2 M Ammonium Tartrate Dibasic, 20% (w/v) PEG 3350 |
