5UMZ

Structure of TNRC6A NLS in complex with importin-alpha

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	78.791, 89.980, 99.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.990 - 1.900
R-factor	0.153
Rwork	0.152
R-free	0.18160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pjn
RMSD bond length	0.016
RMSD bond angle	1.244
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	REFMAC
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.280
High resolution limit [Å]	1.900
Rpim	0.032	0.377
Number of reflections	56250
<I/σ(I)>	19.8
Completeness [%]	100.0
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	sodium citrate, Hepes 7.0, 10mM DTT