5UMV

Crystal structure of the BRCT domain of S. cerevisiae Rev1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2007-10-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9790
Spacegroup name	P 41
Unit cell lengths	33.556, 33.556, 81.838
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.560 - 1.750
R-factor	0.164
Rwork	0.163
R-free	0.19300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vx7
RMSD bond length	0.002
RMSD bond angle	0.496
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (DEV_1106)

Data quality characteristics

	Overall
Low resolution limit [Å]	33.560
High resolution limit [Å]	1.749
Rmerge	0.029
Number of reflections	8356
<I/σ(I)>	55.8
Completeness [%]	91.1
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	Rev1 at 40 mg/mL in 20 mM Tris-HCl, pH 7.0, 50 mM NaCl