5UMS
Crystal structure of middle double PH domain of human FACT complex subunit SSRP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-10-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 63.810, 82.290, 48.505 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.157 - 1.569 |
R-factor | 0.1985 |
Rwork | 0.198 |
R-free | 0.22740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gcl |
RMSD bond length | 0.004 |
RMSD bond angle | 0.623 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (dev_972) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 28.160 |
High resolution limit [Å] | 1.569 |
Number of reflections | 35760 |
<I/σ(I)> | 29.6 |
Completeness [%] | 93.1 |
Redundancy | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 100 mM Bis-Tris, pH 6.5, 28% polyethylene glycol monomethyl ether 2000 |