Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-03-29 |
| Detector | RIGAKU |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.320, 72.420, 61.250 |
| Unit cell angles | 90.00, 109.50, 90.00 |
Refinement procedure
| Resolution | 21.180 - 2.260 |
| R-factor | 0.1498 |
| Rwork | 0.147 |
| R-free | 0.20170 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.417 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (refmac_5.8.0155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 21.180 |
| High resolution limit [Å] | 2.260 |
| Rmerge | 0.051 |
| Number of reflections | 16950 |
| <I/σ(I)> | 2 |
| Completeness [%] | 97.0 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 294 | 18% PEG 8,000, 0.2 M (NH4)2SO4, 50 mM MES, 5 mM DTT, 0.1 mM NaN3, pH 5.9 |
