5ULY
Crystal Structure of Human Protocadherin-15 EC2-3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-21 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.761, 147.625, 77.034 |
| Unit cell angles | 90.00, 101.16, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.640 |
| R-factor | 0.21671 |
| Rwork | 0.215 |
| R-free | 0.24640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4apx 5t4m |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.689 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.640 | 2.640 |
| Rmerge | 0.098 | 0.581 |
| Number of reflections | 42598 | |
| <I/σ(I)> | 10.625 | 1.7 |
| Completeness [%] | 96.4 | 95 |
| Redundancy | 2.6 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277.15 | 0.1 M HEPES (pH 7.5), 0.1 M KCl, 15% PEG 6000 |
