Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.127092 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 108.995, 108.995, 104.659 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.750 - 1.660 |
| R-factor | 0.14084 |
| Rwork | 0.140 |
| R-free | 0.16765 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gvh |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.909 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 37.750 |
| High resolution limit [Å] | 1.660 |
| Number of reflections | 44052 |
| <I/σ(I)> | 14.2 |
| Completeness [%] | 99.6 |
| Redundancy | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 25-28% PEG400, 180-220mM CaCl2, 0.1M Hepes pH 7.5 |
