Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.127092 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 108.995, 108.995, 104.659 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.750 - 1.660 |
R-factor | 0.14084 |
Rwork | 0.140 |
R-free | 0.16765 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gvh |
RMSD bond length | 0.020 |
RMSD bond angle | 1.909 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 37.750 |
High resolution limit [Å] | 1.660 |
Number of reflections | 44052 |
<I/σ(I)> | 14.2 |
Completeness [%] | 99.6 |
Redundancy | 11.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 25-28% PEG400, 180-220mM CaCl2, 0.1M Hepes pH 7.5 |