5ULU

Crystal Structure of Mouse Cadherin-23 EC19-21 (S2087P) with non-syndromic deafness (DFNB12) associated mutation D2148N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	C 1 2 1
Unit cell lengths	84.238, 65.386, 114.651
Unit cell angles	90.00, 98.62, 90.00

Refinement procedure

Resolution	30.337 - 2.850
R-factor	0.19771
Rwork	0.195
R-free	0.24429
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5i8d
RMSD bond length	0.011
RMSD bond angle	1.456
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.850	2.850
Rmerge	0.068	0.181
Rmeas	0.081	0.224
Rpim	0.044	0.130
Number of reflections	14561	556
<I/σ(I)>	15.74	3.375
Completeness [%]	95.7	77.3
Redundancy	3.1	2.3
CC(1/2)		0.970

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.1 M Tris pH 8.5 0.05M MgCl2 25% MPD