5UK9

Wild-type K-Ras(GCP) pH 6.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-11-12
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	39.107, 87.553, 93.692
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.776 - 1.887
R-factor	0.1672
Rwork	0.164
R-free	0.20580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	K-RasQ61H PDB ID 3GFT
RMSD bond length	0.007
RMSD bond angle	0.984
Data scaling software	HKL-3000
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.780	1.960
High resolution limit [Å]	1.887	1.887
Rmerge	0.101	0.389
Number of reflections	26549	1617
<I/σ(I)>	8.72	1.7
Completeness [%]	99.6	97.5
Redundancy	2.2	2
CC(1/2)	0.990	0.797

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	ammonium sulfate, sodium cacodylate, PEG 8000