5UK7
Escherichia coli Hfq bound to dsDNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-07 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 65.749, 65.795, 81.996 |
| Unit cell angles | 105.93, 92.28, 119.92 |
Refinement procedure
| Resolution | 25.685 - 3.000 |
| R-factor | 0.2129 |
| Rwork | 0.210 |
| R-free | 0.25980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gib |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.131 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 8.130 | 3.000 |
| Rmerge | 0.106 | 0.029 | 0.350 |
| Number of reflections | 18997 | ||
| <I/σ(I)> | 9.3 | ||
| Completeness [%] | 83.8 | 95.3 | 53.1 |
| Redundancy | 1.8 | 1.9 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 28-38% MPD, 0.1 M Tris pH 7.5-8.5 |
