5UJS

2.45 Angstrom Resolution Crystal Structure of UDP-N-acetylglucosamine 1-carboxyvinyltransferase from Campylobacter jejuni.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-18
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	C 1 2 1
Unit cell lengths	130.258, 86.024, 80.421
Unit cell angles	90.00, 109.28, 90.00

Refinement procedure

Resolution	29.930 - 2.460
R-factor	0.22661
Rwork	0.224
R-free	0.27456
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5bq2
RMSD bond length	0.009
RMSD bond angle	1.445
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.490
High resolution limit [Å]	2.450	2.450
Rmerge	0.075	0.538
Rmeas	0.045	0.340
Number of reflections	29963	1348
<I/σ(I)>	15.2	2.1
Completeness [%]	98.6	87.7
Redundancy	3.7	3.3
CC(1/2)		0.770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	Protein: 7.7 mg/ml, 0.25M Sodium chloride, 0.01M Tris HCl (pH 8.3); Screen: PEG II (D1), 0.1M Sodium acetate, 0.1 HEPES (pH 7.5), 22% (w/v) PEG 4000