5UJ6
Crystal Structure of Bacteroides Uniformis beta-glucuronidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-17 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.033200 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.728, 142.943, 181.686 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.624 - 1.900 |
R-factor | 0.144 |
Rwork | 0.143 |
R-free | 0.18020 |
Structure solution method | MIR |
RMSD bond length | 0.011 |
RMSD bond angle | 1.276 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.624 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.068 | 0.448 |
Number of reflections | 152573 | |
<I/σ(I)> | 21.1 | 5 |
Completeness [%] | 99.4 | 98.7 |
Redundancy | 10.9 | 11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG 3350, 0.2 M Potassium Chloride |