5UIY
Structure of Bromodomain from human BAZ1A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.432, 44.920, 72.077 |
| Unit cell angles | 105.11, 89.95, 102.92 |
Refinement procedure
| Resolution | 41.286 - 1.687 |
| R-factor | 0.1983 |
| Rwork | 0.196 |
| R-free | 0.23490 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.967 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.286 | 1.760 |
| High resolution limit [Å] | 1.687 | 1.700 |
| Number of reflections | 52361 | |
| <I/σ(I)> | 19.6 | 2.3 |
| Completeness [%] | 95.7 | 86.8 |
| Redundancy | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 100 mM HEPES pH 7.2 and 25% PEG 3350 |
