5UIN
X-ray structure of the W305A variant of the FdtF N-formyltransferase from salmonella enteric O60
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-29 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.467, 59.457, 107.599 |
| Unit cell angles | 90.00, 96.25, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.19078 |
| Rwork | 0.188 |
| R-free | 0.25221 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5uij |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.874 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.095 | 0.264 |
| Number of reflections | 45968 | 5534 |
| <I/σ(I)> | 9.1 | 2.8 |
| Completeness [%] | 98.7 | 96.6 |
| Redundancy | 4.1 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100 mM MOPS 13-16% PEG 3350 200 mL LiCl 5 mM TDP 5 mM folinic acid |
