5UIC
Structure of the Francisella response regulator receiver domain, QseB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-03 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 47.737, 47.737, 191.789 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.250 - 2.487 |
R-factor | 0.226 |
Rwork | 0.225 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kgs |
RMSD bond length | 0.002 |
RMSD bond angle | 0.559 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 38.250 | 50.000 | 2.540 |
High resolution limit [Å] | 2.487 | 6.780 | 2.500 |
Rmerge | 0.116 | 0.070 | 0.862 |
Rmeas | 0.120 | 0.073 | 0.885 |
Rpim | 0.030 | 0.021 | 0.198 |
Number of reflections | 8414 | ||
<I/σ(I)> | 12.2 | ||
Completeness [%] | 99.6 | 96.3 | 99.7 |
Redundancy | 18.1 | 12.7 | 19.6 |
CC(1/2) | 0.996 | 0.990 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1 M potassium citrate, 0.1 M glycine, and 0.05 M bis-tris propane (BTP) pH 7.0 |